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Rapid Diagnosis of Sepsis and Bacterial Meningitis in Children With Real-Time Fluorescent Quantitative Polymerase Chain Reaction Amplification in the Bacterial 16S rRNA Gene

^* To whom correspondence should be addressed. E-mail: qjduan{at}yahoo.com.

	Abstract

a method for the detection of bacterial pathogens in sepsis and bacterial meningitis with 16S rRNa gene– based real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) is developed. a total of 190 blood specimens and 5 cerebrospinal fluid specimens from neonates with suspected sepsis or bacterial meningitis were evaluated with 16S rRNa gene–based real-time FQ-PCR assay. The positive rate of the real-time FQ-PCR assay was significantly higher (25/195, 12.82%) than that of bacterial culture (15/195, 7.69%; P = .002). When bacterial culture was used as a control, the sensitivity of the real-time FQ-PCR was 100%, the specificity was 94.4%, and Youden’s index was 0.944. This study suggests that 16S rRNa gene–based real-time FQ-PCR assay is an important and accurate method in the detection of bacterial pathogens of sepsis and bacterial meningitis and should have a promising usage in the diagnosis of sepsis and bacterial meningitis.

First published on April 30, 2009, doi:10.1177/0009922809333972

Clinical Pediatrics 2009;48:641.

A more recent version of this article appeared on July 1, 2009


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