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Rapid Diagnosis of Sepsis and Bacterial Meningitis in Children with Real-Time Fluorescent Quantitative Polymerase Chain Reaction Amplification in the Bacterial 16S rRNA Gene

Neonatal Department, Children's Hospital, Medical School, Zhejiang University, Hangzhou, China

Qun-Jun Duan, MD

Center Lab, Children's Hospital, Medical School, Zhejiang University, Hangzhou, China

Mei-Ting Cai, MD

Center Lab Children's Hospital, Medical School, Zhejiang University, Hangzhou, China

Yi-Dong Wu, MD

Center Lab Children's Hospital, Medical School, Zhejiang University, Hangzhou, China, qjduan{at}yahoo.com

Shi-Qiang Shang, MD

Center Lab Children's Hospital, Medical School, Zhejiang University, Hangzhou, China

A method for the detection of bacterial pathogens in sepsis and bacterial meningitis with 16S rRNA gene— based real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) is developed. A total of 190 blood specimens and 5 cerebrospinal fluid specimens from neonates with suspected sepsis or bacterial meningitis were evaluated with 16S rRNA gene—based real-time FQ-PCR assay. The positive rate of the real-time FQ-PCR assay was significantly higher (25/195, 12.82%) than that of bacterial culture (15/195, 7.69%; P = .002). When bacterial culture was used as a control, the sensitivity of the real-time FQ-PCR was 100%, the specificity was 94.4%, and Youden's index was 0.944. This study suggests that 16S rRNA gene—based real-time FQ-PCR assay is an important and accurate method in the detection of bacterial pathogens of sepsis and bacterial meningitis and should have a promising usage in the diagnosis of sepsis and bacterial meningitis.

Key Words: sepsis • bacterial meningitis • polymerase chain reaction • diagnosis • bacterial culture

This version was published on July 1, 2009

Clinical Pediatrics, Vol. 48, No. 6, 641-647 (2009)
DOI: 10.1177/0009922809333972


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