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Use of an Anaerobic Culture Jar in Processing Pediatric Throat Cultures

Department of Pediatrics, University of Virginia Medical Center, Charlottesville, Virginia

Sharon Dudley

Department of Pediatrics, University of Virginia Medical Center, Charlottesville, Virginia

J. Owen Hendley

Department of Pediatrics, University of Virginia Medical Center, Charlottesville, Virginia

Two hundred consecutive throat cultures from a Pediatric Walk-in Clinic were processed, using both aerobic and anaerobic culture techniques. The 35 aerobic isolates of group A beta-hemolytic streptococci (BHS) were all confirmed anaerobically as well, and there were two additional isolates detected only by the anaerobic technique. Of these 37 bacitracin-sensitive isolates, only 24 (65%) could be identified at 24 hours using the aerobic technique, compared to 29 (78%) using the anaerobic technique.

In contrast to this relatively small effect upon the isolation of group A BHS, the yield of BHS from groups B, C, F, and G was more than doubled by the anaerobic technique. The confusion engendered by the improved detection of these bacitracin-resistant BHS using the anaerobic tech nique offset the small advantage in thoroughness and speed of detection of group A organisms. On this account, until more is known about the possible signaficance of isolating nongroup A BHS in the pharynx, the advisability of using an anaerobic culture jar to process pediatric throat cultures remains uncertain.

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